Introduction
Methods
Subjects & sub-grouping
Laboratory tests
Statistical analysis
Results
Basic demographic and clinical characteristics
Variables | FT (n = 41) | FT + LP (n = 42) | FT + HP (n = 42) |
P -value
|
---|---|---|---|---|
Gestational age (weeks)
| 28.32 ± 2.88 | 27.66 ± 2.91 | 27.81 ± 2.46 |
0.82
1
|
Body weight (g)
| 1090.85 ± 399.33 | 1017.90 ± 368.30 | 1020.83 ± 288.04 |
0.57
3
|
Delivery type (NSVD / CS)
| 9 / 32 | 13 / 29 | 7 / 35 |
0.29
1
|
Sex (male / female)
| 19 / 22 | 20 / 22 | 24 / 18 |
0.56
1
|
Small for gestational age
| 13 (31.7) | 9 (21.4) | 11 (26.2) |
0.58
3
|
Surfactant use
| 31 (75.6) | 35 (83.3) | 36 (85.7) |
0.46
1
|
Bronchopulmonary dysplasia
| 21 (51.2) | 25 (59.5) | 28 (66.7) |
0.36
1
|
Significant patent ductus arteriosus*
| 18 (43.9) | 20 (47.6) | 16 (38.1) |
0.67
1
|
Retinopathy of prematurity (≥ stage 2)
| 7 (17.1) | 8 (19.0) | 11 (26.2) |
0.56
1
|
Necrotizing enterocolitis
| 3 (7.3) | 5 (11.9) | 9 (21.4) |
0.16
1
|
Sepsis
| 12 (29.3) | 17 (40.5) | 18 (42.9) |
0.40
1
|
Total parental nutrition days
| 34.37 ± 23.04 | 41.31 ± 25.44 | 47.52 ± 35.31 |
0.20
4
|
Days to achieve full enteral feeding
| 37.66 ± 33.04 | 43.02 ± 29.67 | 44.31 ± 31.49 |
0.30
4
|
Average HM intake (mL/day)
| 86.94 ± 62.65 | 82.15 ± 59.78 | 75.43 ± 50.04 |
0.93
4
|
Proportion of HM in total enteral intake
| ||||
< 25% | 4 (9.8) | 5 (11.9) | 7 (16.7) |
0.76
1
|
25 – 49% | 17 (41.5) | 12 (28.6) | 14 (33.3) | |
50 – 75% | 11 (26.8) | 11 (26.2) | 12 (28.6) | |
> 75% | 9 (22.0) | 14 (33.3) | 9 (21.4) | |
Duration of HM feeding (days)
| 40.46 ± 17.91 | 41.29 ± 18.61 | 42.46 ± 20.03 |
0.58
4
|
Numbers of transfusion
| ||||
Packed red blood cell | 6.07 ± 8.60 | 9.33 ± 12.00 | 6.81 ± 7.61 |
0.67
4
|
Platelet concentrate | 2.22 ± 4.71 | 6.38 ± 14.36 | 2.55 ± 5.49 |
0.56
4
|
Fresh frozen plasma | 1.10 ± 3.40 | 2.67 ± 12.30 | 0.62 ± 1.06 |
0.67
4
|
CMV infection
| 2 (4.9) | 4 (9.5) | 1 (2.4) |
0.40
2
|
Outcome (survival / death)
| 40 / 1 | 39 / 3 | 41 / 1 |
0.41
1
|
Comparison of infants according to CMV infection
Variables | CMV infection | Univariate logistic regression analysis | ||||||
---|---|---|---|---|---|---|---|---|
Yes (n = 7) | No (n = 118) |
P-value
| Odds ratio (95% CI) |
P-value
| ||||
Gestational age (weeks)
| 27.86 ± 3.44 | 27.73 ± 2.68 |
0.63
2
| 0.97 (0.71–1.31) |
0.81
| |||
Body weight (g)
| 1012.86 ± 271 | 1044.59 ± 358 |
0.82
2
| 1.00 (1.00–1.00) |
0.82
| |||
Delivery type (NSVD / CS)
| 3 / 4 | 26 / 92 |
0.35
1
| 0.38 (0.08–1.79) |
0.22
| |||
Sex (male / female)
| 2 / 5 | 61 / 57 |
0.27
1
| 2.68 (0.50-14.34) |
0.25
| |||
Small for gestational age
| 1 (14.3) | 20 (16.9) |
1.00
1
| 0.79 (0.14–4.56) |
0.80
| |||
Type of pregnancy
| ||||||||
Single / twin or triplet
| 6 / 1 | 47 / 71 |
0.04
1
| 0.11 (0.01–0.95) |
0.04
| |||
Surfactant use
| 6 (85.7) | 96 (81.4) |
1.00
1
| 1.37 (0.16–12.01) |
0.77
| |||
Bronchopulmonary dysplasia
| 4 (57.1) | 70 (59.3) |
1.00
1
| 0.91 (0.20–4.27) |
0.91
| |||
Significant patent ductus arteriosus*
| 3 (42.9) | 51 (43.2) |
1.00
1
| 0.99 (0.21–4.60) |
0.98
| |||
ROP (≥ stage 2)
| 2 (28.6) | 24 (20.3) |
0.63
1
| 1.57 (0.29–8.58) |
0.60
| |||
Necrotizing enterocolitis
| 0 (0.0) | 17 (14.4) |
0.59
1
| N/E | ||||
Sepsis
| 2 (28.6) | 45 (38.1) |
0.71
1
| 0.65 (0.12–3.49) |
0.61
| |||
Numbers of transfusion
| ||||||||
Packed red blood cell | 11.43 ± 11.47 | 7.18 ± 9.50 |
0.38
1
| 1.04 (0.97–1.11) |
0.27
| |||
Platelet concentrate | 5.29 ± 9.39 | 3.64 ± 9.46 |
0.97
1
| 1.01 (0.95–1.08) |
0.66
| |||
Fresh frozen plasma | 0.43 ± 0.79 | 1.53 ± 7.62 |
0.74
1
| 0.83 (0.39–1.76) |
0.62
| |||
Total parental nutrition days
| 47.57 ± 28.59 | 40.74 ± 28.83 |
0.42
3
| 1.01 (0.98–1.03) |
0.54
| |||
Days to achieve full enteral feeding
| 44.14 ± 31.68 | 41.55 ± 31.40 |
0.69
3
| 1.00 (0.98–1.03) |
0.83
| |||
Average HM intake (mL/day)
| 80.55 ± 45.74 | 81.51 ± 58.21 |
0.70
3
| 1.00 (0.99–1.01) |
0.97
| |||
Proportion of HM in total enteral intake
| ||||||||
< 25% | 1 (14.3) | 15 (12.7) | 0.331 | Reference | ||||
25% – 49% | 1 (14.3) | 42 (35.6) | 0.36 (0.02–6.08) |
0.48
| ||||
50% – 75% | 4 (57.1) | 30 (25.4) | 2.00 (0.21–19.50) |
0.55
| ||||
> 75% | 1 (14.3) | 31 (26.3) | 0.48 (0.03–8.28) |
0.62
| ||||
Study group
| ||||||||
FT | 2 (28.6) | 39 (33.1) |
0.40
1
| Reference | ||||
FT + LP | 4 (57.1) | 38 (32.2) | 2.05 (0.35–11.87) |
0.42
| ||||
FT + HP | 1 (14.3) | 41 (34.7) | 0.48 (0.04–5.46) |
0.55
|
Quantitative analysis of CMV DNA in HM
FT |
p-
| FT + LP |
p-
| FT + HP |
p-
|
overall
| ||||
---|---|---|---|---|---|---|---|---|---|---|
Lsmean | SE |
value
| Lsmean | SE |
value
| Lsmean | SE |
value
|
p-value
| |
In case of using all data | ||||||||||
Fresh | 2.354 | 0.314 | 2.871 | 0.312 | 2.565 | 0.283 |
0.9782
| |||
Post | 2.287 | 0.314 | 2.681 | 0.312 | 2.122 | 0.283 | ||||
Difference | 0.067 | 0.078 |
0.400
| 0.190 | 0.066 |
0.01
| 0.443 | 0.064 |
< .0001
|
0.001
|
In case of using only 9 and 12 weeks data | ||||||||||
Fresh | 2.493 | 0.452 | 3.257 | 0.366 | 2.905 | 0.328 |
0.7308
| |||
Post | 2.484 | 0.449 | 2.944 | 0.367 | 2.293 | 0.329 | ||||
Difference | 0.010 | 0.098 |
0.9225
| 0.313 | 0.072 |
0.0002
| 0.612 | 0.071 |
< .0001
|
0.0001
|
No. | GA (weeks), BW (gram) | Sex | Number of transfusions (PRBC / PC / FFP) | Study group | Age at diagnosis (PCA) | CMV-positive specimen | Average HM intake (mL/day) | Symptom | Duration of gancyclovir treatment | Outcome |
---|---|---|---|---|---|---|---|---|---|---|
1 | 25 + 2, 720 | F | 17 / 0 / 0 | FT + LP | 28 (29 + 2) | Blood, urine, BAL, CSF | 71 | Lung | 3 weeks | Survived |
2 | 28 + 6, 1210 | M | 6 / 0 / 0 | FT + HP | 28 (32 + 6) | Urine | 89 | None | 2 weeks | Survived |
3 | 27 + 2, 1040 | F | 25 / 23 / 0 | FT + LP | 29 (31 + 3) | Blood, urine, sputum | 11 | Lung, SLS (PLT↓, AST/ALT↑) | 3 weeks | Died |
4 | 26 + 6, 1080 | F | 5 / 0 / 6 | FT | 83 (39 + 2) | Urine | 153 | None | N/A | Survived |
5 | 24 + 0, 630 | M | 27 / 14 / 2 | FT + LP | 56 (32 + 0) | Urine | 104 | None | N/A | Survived |
6 | 33 + 4, 1420 | F | 0 / 0 / 0 | FT + LP | 28 (37 + 4) | Urine | 41 | None | N/A | Survived |
7 | 32 + 6, 990 | F | 0 / 0 / 0 | FT | 56 (40 + 6) | Urine | 94 | None | N/A | Survived |