Erschienen in:
01.12.2024 | Original Article
Laser-photobiomodulation on reactive oxygen species production in titanium disc-containig pre-osteoblast cells culture
verfasst von:
Guilherme Aparecido Monteiro Duque da Fonseca, Igor da Silva Brum, Letícia Fernandes Dezembro Ramos, Juliana Azevedo Marques Gaschler, Lucio Frigo
Erschienen in:
Lasers in Dental Science
|
Ausgabe 1/2024
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Abstract
Purpose
The therapeutic success of dental implants depends on the wound healing process. Bone matrix production by osteoblast cells around implant is pivotal in this process. Osteoblast cells reactive oxygen species (ROS) production and cell adhesion are the initial steps in osseointegration. The Objective the aim was to investigate the laser-photbiomodulation (L-PBM) effect (GaAlAs, λ = 830 nm) on ROS production and membrane lipid peroxidation (LPO) in osteoblast cells cultures seeded on titanium (Ti) disc-containing medium. Cell cultures.
Methods were divided into six groups
Control group: without Ti disc containing-medium and Ti disc-containing groups, that were divided in four distinct subgroups: irradiated with an energy density of 2 J/cm2, 4 J/cm2, 6 J/cm2, 8 J/cm2, at irradiation intervals of 24, 48 and 72 h.
Results
After irradiation, groups were evaluated on: 1- quantification of H2O2 and LPO; 2- photomicrographic record of cell adhesion/proliferation. Photomicrographs showed that L-PBM increased cell adhesion to Ti disc. There was an increase of H2O2 production in a dose-dependent manner and a decrease in LPO levels after 48 h.
Conclusion
Results indicate that L-PBM stimulates the production of H2O2, and this increase has no effect on the peroxidation of membrane lipids. L-PBM may be related to the enhancement of osteoblast adhesion to Ti disc surface.