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Pediatric Surgery International

Erschienen in:

22.08.2022 | Original Article

Intrauterine exposure to oxidative stress induces caspase-1-dependent enteric nerve cell pyroptosis

verfasst von: Lingling Zhou, Bingyu Wang, Hua Xie, Chunxia Du, Jie Tang, Weibing Tang

Erschienen in: Pediatric Surgery International | Ausgabe 11/2022

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Abstract

Purpose

This study determined whether oxidative stress causes the developmental abnormalities of the enteric nervous system during the embryonic period.

Methods

Using the test results of tissue specimens of children with Hirschsprung disease (HSCR), we established a pregnant rat model of oxidative stress and a cellular oxidative stress model to conduct related molecular, cellular, and histopathological experiments for exploration and validation.

Results

The results of the quantitative real-time polymerase chain reaction assay indicated overexpression of pyroptosis markers (NLRP3, ASC, and caspase-1) in HSCR lesions and newborn pups in the oxidative stress group (treated with d-galactose). The expression of cathepsin D was significantly decreased in intestinal tissues of newborn pups in the oxidative stress group compared to the control group. Reactive oxygen species scavengers (N-acetyl-cysteine, NAC), the caspase-1 inhibitor (VX-765), and the NLRP3 siRNA could reverse the release of LDH, decrease the number of propidium iodide stained cells, and reduce the percentage of TUNEL/caspase-3 double-positive cells in the H₂O₂-treated group.

Conclusion

Oxidative stress can induce the death of enteric nerve cells by activating caspase-1-dependent pyroptosis through NLRP3 inflammasomes, which may contribute to abnormal enteric nervous system development.

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Titel: Intrauterine exposure to oxidative stress induces caspase-1-dependent enteric nerve cell pyroptosis
verfasst von: Lingling Zhou
Bingyu Wang
Hua Xie
Chunxia Du
Jie Tang
Weibing Tang
Publikationsdatum: 22.08.2022
Verlag: Springer Berlin Heidelberg
Erschienen in: Pediatric Surgery International / Ausgabe 11/2022
Print ISSN: 0179-0358
Elektronische ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-022-05199-8

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Intrauterine exposure to oxidative stress induces caspase-1-dependent enteric nerve cell pyroptosis

Abstract

Purpose

Methods

Results

Conclusion

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