Effect of interleukin-1 antagonist on growth of children with colchicine resistant or intolerant FMF

Familial Mediterranean Fever (FMF) represents the most common form of monogenic autoinflammatory disease [1]. It is characterized by recurrent, self-limited, inflammatory attacks, lasting 1–3 days. FMF results from gain of function mutations in the MEFV gene located on chromosome 16 which encodes pyrin, a 781-amino acid long protein expressed primarily in cells of the innate immune system. There, it serves as a negative regulator of a cytosolic cellular multiprotein complex termed the inflammasome, which mediates the activation of various inflammatory pathways in response to deleterious cellular insults (e.g., infection) and drives the maturation of the IL-1b and IL-18. Accordingly, dysregulated inflammasome activity results in a pro-inflammatory state and has been linked to autoinflammatory diseases [2]. In FMF, the added pyrin activity leads to an unrestricted inflammasome activation, thereby resulting in a hyperinflammatory state [2, 3]. Among the different mutations described in MEFV, the most common is M694V, which has been shown to present in 20–65% of patients. Importantly, M694V homozygotes suffer from a more severe form of the disease, including frequent episodes and the need for higher colchicine doses to prevent attacks, as compared to patients with other mutations [4]. Approximately 10–20% of individuals who meet diagnostic criteria for FMF have no identified mutations in the MEFV gene [5]. Colchicine, which still represents the mainstay of FMF treatment, has been shown to prevent FMF attacks in 60–65% of patients and to result in a partial remission in an additional 30–35% of patients [6]. Moreover, colchicine is capable of eliminating the long-term risk of secondary amyloidosis, which is the most significant complication in FMF. An additional complication of FMF in the pediatric population is the negative impact on linear growth [7], similar to observations in other chronic diseases in children [8, 9]. Indeed, it has been shown that successful treatment with colchicine has a positive effect on both height and weight parameters in children with FMF [10‐12]. These studies suggest that colchicine improves growth by suppressing disease activity and inflammation. Yet, up to 10% of FMF patients do not respond to colchicine treatment, even under maximal doses and full compliance [13]. In recent years, a growing understanding of the molecular basis behind FMF has resulted in a new generation of drugs, directed against IL-1. These include anakinra, which is a recombinant form of the naturally occurring IL-1 receptor antagonist [14], and canakinumab, a high affinity, fully human monoclonal anti-IL-1b antibody [15]. Both drugs are currently prescribed to colchicine-resistant or -intolerant FMF patients, although colchicine is still used in these patients to prevent amyloidosis, as the two biological drugs have not been consistently shown to have this effect. Both anakinra and canakinumab have a relatively favorable safety profile, with the most common side effects being injection site reactions and infections (typically of the upper respiratory tract) [16, 17]. Despite the growing use in anti-IL1 drugs in FMF among children, data is still lacking in regard to long-term follow up. In addition, the information regarding the impact of these novel drugs on linear growth in children with FMF is minimal [18]. Herein, we carried out a single center retrospective cohort study involving 22 pediatric FMF patients treated with anti-IL1 agents to evaluate their long-term growth response to treatment in addition to assessment the overall efficacy and safety of these agents.

Anti-IL-1 agents are highly effective and safe in patients with colchicine-resistant FMF [17, 21, 22]. However, the efficacy and safety profile of these drugs in the treatment of pediatric FMF is largely limited to a relatively short duration of follow-up. To the best of our knowledge, this study provides efficacy and safety data from the longest follow-up to date, with reassuring evidence that this group of drugs is extremely well tolerated and results in excellent clinical outcomes, with 80% achieving complete treatment response.

The main finding of our study is the significant increase in both height and body weight percentiles among FMF patients treated with anti-IL-1 drugs. Our results corroborate with historical reports demonstrating a positive effect of colchicine on growth and development in pediatric FMF patients [10‐12]. Of interest, one study demonstrated that an earlier initiation of colchicine resulted in a larger positive effect on linear growth [12]. This finding could be explained by an earlier alleviation of the deleterious effects of chronic diseases in general and inflammatory disorders in particular, on linear growth [23, 24].

Two previous studies reported the influence of canakinumab on linear growth in autoinflammatory conditions in children. In the study of Balci et al. of 11 children with FMF, despite a median follow-up of only 1.5 years, canakinumab treatment had a positive effect on both height and body weight Z-scores [18]. Yücel et al., however, have failed to demonstrate a positive effect of canakinumab on linear growth and reported an improvement in body weight Z-scores only [17]. This lack of improvement in height percentiles in this study was attributed by the authors for late initiation of treatment and a relatively short duration of follow-up. Taken together with our results, one may argue in favor of early initiation of anti-IL-1 agents in colchicine resistant FMF patients not only for better control of inflammatory attacks but also to maximize their linear growth potential.

There are different reports in the literature regarding the percentage of patients who had complete response to anti IL-1 agents. Complete remission following canakinumab treatment was reported by several groups, such as Brik et al. who reported 42.9% with complete response [25], De Benedetti et al. [26], and Kisla Ekinci et al. [27] reporting both of ~ 70% with complete response, with some reporting a complete response in up to 100% of cases [22, 28]. This difference could be related to variation in treatment regimen between studies and distribution of MEFV mutations in the various cohorts.

Interestingly, in this study we report a very low rate of side effects, despite a mean follow-up of more than 3 years. One patient reported an injection site skin reaction, and two additional patients complained of abdominal pain. These results are in-line with published data regarding canakinumab safety [17, 22, 27]. It is worth noting that in a randomized, placebo-controlled trial of canakinumab in autoinflammatory diseases in children, a somewhat higher rate of adverse events was recorded, including infections (23.8%), injection site reactions, (6.0%), headache (3.9%) and abdominal pain (3.6%) [26]. In particular, infections have previously been reported in some studies to occur in patients treated with anti-IL-1 drugs, including major bacterial infections (e.g. pneumonia [20, 29]). Nevertheless, in our study, none of the patients reported of significant infections during their routine follow-up visits. In addition, none of our patients discontinued the treatment due to side effects, similar to what was described in a systematic review of 19 studies of canakinumab [21].

As for the genetic profile of pediatric FMF children, our findings are consistent with the more severe phenotype described in patients who are homozygous for the M694V mutation [30]. We found that 95% of FMF patients who required the use of anti-IL-1 agents were homozygous to the M694V mutation, as opposed to only 30.5% in the entire genetic cohort at our Center. In fact, 12.6% of the patients harboring the M694V homozygous mutation were eventually treated with anti-IL-1. Thus, patients with severe disease course who are not adequately controlled by colchicine, especially those who are homozygotes for the M694V mutation, should be considered for an early intervention with anti-IL1 agents, in order to gain maximal benefits of this treatment regimen on linear growth.

This study has several limitations that require consideration. First, the retrospective design does not allow us to infer on causality. Second, the relatively small number of patients limits our ability to address the age and sex specific effects of anti-IL-1 drugs on growth. Yet, our comprehensive database with detailed clinical parameters of over 600 FMF patients treated at our institution and a mean follow-up of over 3 years provide us with a unique opportunity to conclude regarding the long-term anthropometric effects of anti-IL-1 agents in children with FMF.

Our study shows that anti -IL-1 agents represent an effective and safe treatment and may improve growth in children with colchicine-resistant FMF. Therefore, we suggest considering early treatment when needed, especially for those who harbor the M694V homozygous mutation.