Clinical characteristics and outcomes of chronic nonbacterial osteomyelitis in children: a multicenter case series

Chronic nonbacterial osteomyelitis (CNO) is a benign and noninfectious autoinflammatory bone disease that mostly affects children and adolescents [1‐3]. In 1972, Giedion et al. first described four patients with subacute or chronic multifocal symmetrical osteomyelitis, which mostly affects the growth plates of long bones [4]. Some scholars subsequently described similar cases and gave the diagnostic term “chronic recurrent multifocal osteomyelitis (CRMO)” [5]. Recurrent flares of inflammatory bone pain related to aseptic osteomyelitis are the major symptoms of the disease. The clinical presentation varies widely, from mild, unifocal, and time-limited bone involvement to severe, chronically active, or recurrent disease with multifocal bone lesions [5‐8]. Thus, the name “CNO” has been proposed to encompass them all [9]. In adults, the term SAPHO syndrome is commonly used when referring to manifestations of the disease, which include synovitis, acne, pustulosis, hyperostosis, and osteitis [10]. To date, whether CNO and SAPHO syndrome are different manifestations of the same disease at different ages of onset or different outcomes of different clinical manifestations of the same disease remains unresolved [11].

Epidemiological data on CNO from Chinese population are limited. The average age at onset of the disease is 7–12 years, but the delay in diagnosis from the onset of symptoms is usually around 1 year [12]. A study conducted in Germany reported an incidence of 0.4 per 100,000 children [13]. The current incidence of the disease in other regions is unknown. The true incidence of CNO has likely been underestimated in past studies [14]. In most multicenter studies from North America and Europe, there is a female predominance with a female:male ratio of approximately 2:1. However, in similar studies from India and Japan, a male predominance is observed [8, 12]. To date, CNO is usually diagnosed by exclusion with magnetic resonance imaging (MRI) and/or through bone biopsy, which reveals chronic inflammation without infectious and oncological agents. The treatment protocol for CNO includes NSAIDs, steroids, MTX, SASP, TNF-α inhibitors, and bisphosphonates.

Although the awareness of CNO has increased over the past decade, misdiagnosis and delays in treatment still persist. Reliable diagnostic criteria and treatment protocols are lacking, and more multicenter studies on CNO are needed. To improve our understanding of the disease, we sought to evaluate the demographic and clinical characteristics, radiological findings, as well as treatment responses in pediatric patients with CNO at five tertiary centers in South China.

Patients under 18 years old diagnosed with CNO between 2014 and 2021 were enrolled at five tertiary centers in South China: Children’s Hospital of Nanjing Medical University, Children’s Hospital of Fudan University, Children’s Hospital of Soochow University, Children’s Hospital of Zhejiang University School of Medicine, and Children’s Hospital of Chongqing Medical University. The study was approved by local Ethics Review Board, and granted exemption of informed consent. Clinical characteristics, such as age, sex, family history, age at onset of symptoms, delay in diagnosis, symptoms, comorbidities, and treatments, were recorded. Available laboratory evaluation images, including plain radiographs, computed tomography (CT), Tc99 bone scan, MRI, and histology from bone biopsy were collected. Literature review was conducted on the case series of CNO reported abroad, and clinical comparison was made among our study and foreign CNO cohorts.

The diagnosis of CNO was defined as the presence of unifocal or multifocal inflammatory bone lesions with radiological and/or histopathological characteristics compatible with this diagnosis [15]. Infectious, oncological, or other inflammatory diseases were excluded. Response to treatment was assessed by improvement in pain and serologic markers of inflammation as well as radiographic proof of bone healing. The pain was evaluated by Visual Analogue Scale/Score. “No response” was defined as persistent pain with elevated inflammatory markers and abnormal signal on MRI.“Complete response/Remission” was defined as an 80% or more remission of clinical symptoms and imaging manifestation as well as normal inflammatory markers. The remission of 20% ~ 80% is “partial response”.

The present study was a multicenter series of CNO patients from five medical centers in South China. Compared with previous reports, the clinical characteristics of our patients were a little different. (details in Table 4) [8, 12, 15, 17‐25] The female advantage in China is not such obvious as European countries [8, 12, 15, 18, 22‐25]. The median age at diagnosis and diagnostic delays were 9.2 years and 10.2 months, respectively. The diagnostic delay ranged from 1 to 72 months, suggesting that CNO is still sometimes not well recognized in our country. In term of clinical manifestation, bone pain is still the most frequency symptom, but the fever rate in our study, which up to 44.4%, is more higher than that in other studies [17, 18, 20, 22, 24]. This may be related to bone inflammation. The most common bones involved in our study is the long bones of the limbs, especially the lower bones. In cases of Europe and USA, the clavicle, pelvis and spine are more frequently involved [8]. The comorbidities of our patients are mainly arthritis, only one patient has acne and gastrointestinal ulcer. The gastrointestinal symptoms, palmoplantar pustulosis, psoriasis and acne are more frequently in European cases [8, 12, 20]. It is also different from other studies, our CNO patients had no family history, no HLA-B27 positivity and no ANA positivity, suggesting a different genetic background.

On physical examination, swelling is infrequently observed, but when the disease is active, it is a sign of specific points of bone sensitivity [9]. CNO most frequently involves the long bones, followed by the pelvic bones, the vertebral column or the shoulder girdle/clavicle [3, 26]. The bones involved tend to be symmetrical, except the clavicle. Unifocal long bone involvement needs to be distinguished from culture-negative bacterial osteomyelitis by blood bacterial culture or bone marrow [27].

Laboratory tests of CRMO are not specific. Routine inflammatory parameters (WBC, white blood cell count; CRP, C reactive protein; ESR, erythrocyte sedimentation rate) are usually normal or mildly elevated. Imaging techniques are vital for diagnosing CNO and for excluding differential diagnoses [28]. Of our patients, 94.5% were evaluated with MRI, 52.9% with X-ray, and 58.5% with CT, while 64.7% had a bone scintigraphy. Compared with X-ray and CT, MRI is the most sensitive imaging technique to determine the extent and severity of bone involvement, particularly in the early stage. They can detect bone edema even before bone erosions and sclerosis develop and help assess the inflammation of surrounding tissues [29, 30]. More recently, whole-body MRI has been reported to be useful to screen the entire skeleton for bone lesions [31]. Bone scintigraphy is also useful for this purpose. It provides a global skeletal assessment at a lower cost [32], which can show abnormal concentrations of radionuclides, indicating the site of lesions, but cannot distinguish between inflammation and bone marrow metabolic hyperplasia. The radiation is also harmful to the body.

Bone biopsies are usually performed to exclude chronic infections, malignancies, or other systemic diseases, especially in patients with unifocal lesions [33]. In the present study, 47.1% of our patients underwent a bone biopsy. The histopathological findings of CNO are nonspecific inflammatory changes. A bone biopsy followed by pathological and pathogenic examination is very helpful for differential diagnosis.

To help diagnosis, we used the clinical NBO scores provided by Annette F. Jansson et al. [16] Although the score provides a reference standard for the diagnosis of the disease, CNO continues to be a diagnosis of exclusion. Important differential diagnoses include malignancies, infections, immunodeficiency, Langerhans cell histiocytosis (LCH), and other autoinflammatory disorders [33].

Although three consensus treatment plans (CTPs) were developed for CNO patients refractory to NSAID monotherapy by the Childhood Arthritis and Rheumatology Research Alliance (CARRA) [34], therapy protocols of CNO are not yet standardized. In general, first-line treatment is NSAIDs, which may reduce the pain and, in some cases, decrease the number of bone lesions in 3 months [35]. Second-line treatments usually includes methotrexate, corticosteroids, biologic drugs (mainly TNF-α inhibitors), and bisphosphonates depending on the severity of the disease and the presence of comorbidity and/or complications [36]. Almost all of our patients started treatment with NSAIDs but had to switch to other treatments because of partial response and relapse. Methotrexate was the second most frequent treatment, but six of these seven patients received steroids, bisphosphonates, or biologic drugs at the same time. Thus, it is difficult to assess the real impact of methotrexate. Different biologicals have been used to treat CNO, most commonly TNF-α inhibitors [25]. In our study, remission was achieved more frequently with TNF-α inhibitors, including adalimumab and etanercept. Bisphosphonates given to seven patients, resulted in remission in six patients. One patient experienced worsened bone pain after bisphosphonates, subsequently remitting on an adalimumab and bisphosphonate combination, which achieved a good result. Based on our clinical experiences, long bone lesions in diaphyses are more easily improved than those in epiphyses. It is worth noting that, spinal involvement can lead to fractures and secondary bone deformity [37]. This emphasizes the need for early diagnosis and aggressive treatment to prevent complications. Despite recent advances, there is no information on the optimal duration of treatment. Further studies about treatmnt are needed.

The long-term prognosis of CNO is generally favorable, with remission observed in 40% of patients after 1–5 years of follow-up [38]. In our study, we had a remission rate of 11.7% without medicines in a median follow-up of 16 months. The recurrence of the disease is very common. In a US cohort, a recurrence rate of 83% was observed after a follow-up of 1.8 years [21]. In our study, the recurrent rate was 17.6%. This may be related to the small sample size. It has been reported that patients can present a flare even 15 years after the onset of the disease, so it requires monitoring and long-term follow-up [38].

This study is the first case series of CNO from South China to describe the features and outcomes of such an autoinflammatory bone disease. The diagnosis should include clinical history, laboratory and imaging examination, and histopathological examination. Other causes of chronic bone pain should be ruled out. For treatment, NSAIDs are used as first-line drugs followed by steroids, MTX, SASP, bisphosphonates, and TNF-α inhibitors. Combination therapy with bisphosphonates and TNF-α inhibitors may be an option for refractory CNO. The limitation of this study is its small sample size. Thus, further studies including more patients from other tertiary centers are required to formulate diagnostic and treatment strategies for CNO.