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09.06.2023 | Original Article

Autophagy in an extruded disc compared to the remaining disc after lumbar disc herniation in the same patient

verfasst von: Jun-Yeong Seo, Jinu Kim, Young-Yul Kim, Kee-Yong Ha, Young-Hoon Kim, Sang-Il Kim, Jae-Hak Lim, Kyu Bum Seo, Hyunseong Kang, Sungwook Choi, Md Abdul Khaleque

Erschienen in: European Spine Journal | Ausgabe 1/2024

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Abstract

Purpose

The purpose of this study was to investigate autophagy in an extruded disc and to compare this activity with the activity in the remaining disc after lumbar disc herniation in the same patient.

Methods

In total, 12 patients (females 4, males 8) with the extruded type of lumbar disc herniation (LDH) were surgically treated. Their mean age was 54.3 ± 15.8 years (range: 29 ~ 78 years). The mean interval from the occurrence of symptoms to the operation was 9.8 ± 9.4 weeks (range: 2 ~ 24 weeks). The extruded discs were excised, and the remaining disc material removed, to prevent recurrence of herniation. Immediately after specimen collection, all tissues were stored at −70 °C prior to analysis. Autophagy was assessed immunohistochemically and via Western blotting for Atg5, Atg7, Atg12, Atg12L1, and Beclin-1. And the relationship between autophagy and apoptosis was investigated by correlation analysis of caspase-3 with autophagy proteins.

Results

The expression levels of autophagic markers were significantly increased in the extruded discs compared to the remaining discs within the same patients. The mean expression levels of Atg5, Atg7, Atg12, and Beclin-1 in extruded discs were statistically significantly higher than those in the remaining discs (P < 0.01, P < 0.001, P < 0.01, and P < 0.001 respectively).

Conclusions

The autophagic pathway was more active in extruded disc material than in remaining disc material within the same patient. This may explain spontaneous resorption of the extruded disc after LDH.

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Titel: Autophagy in an extruded disc compared to the remaining disc after lumbar disc herniation in the same patient
verfasst von: Jun-Yeong Seo
Jinu Kim
Young-Yul Kim
Kee-Yong Ha
Young-Hoon Kim
Sang-Il Kim
Jae-Hak Lim
Kyu Bum Seo
Hyunseong Kang
Sungwook Choi
Md Abdul Khaleque
Publikationsdatum: 09.06.2023
Verlag: Springer Berlin Heidelberg
Erschienen in: European Spine Journal / Ausgabe 1/2024
Print ISSN: 0940-6719
Elektronische ISSN: 1432-0932
DOI: https://doi.org/10.1007/s00586-023-07731-3

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Autophagy in an extruded disc compared to the remaining disc after lumbar disc herniation in the same patient